The figures are representative of three independent experiments. Supplementary information Supplemental Information(16K, docx) Physique S1(3.8M, tif) Physique S2(4.6M, tif) Acknowledgements We would like Ursocholic acid to thank Prof. but constantly homing mDCs. test for two groups or analysis of variance for multiple groups. * represents P?P?P?CXCL12 significance. The figures are representative of three impartial experiments. Supplementary information Supplemental Information(16K, docx) Physique S1(3.8M, tif) Physique S2(4.6M, tif) Acknowledgements We would like to thank Prof. Yu Zhang (Department of Immunology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, P.R. China) and Prof. Xiaoren Zhang (Affiliated Cancer Hospital and Ursocholic acid Institute of Guangzhou Medical University, Guangzhou Municipal and Guangdong Provincial Key Laboratory of Protein Modification and Degradation, State Key Laboratory of Respiratory Disease, Guangzhou, P. R. China) for guidance and help. We thank Prof. Weifeng Chen (Department of Immunology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, P. R. China) and Xiaojie Zhang (Department of Neurology, University of Michigan, Ann Arbor, Michigan, USA) for the nice gifts of mTEC1 (a medullary-type mouse thymic epithelial cell line 1), L cells (the fibroblast cell line without Jagged1 expression) and J cells (a Jagged1 gene stably transfected cell line), respectively. We thank Dr. Rong Jin (Department of Immunology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, P. R. China) for training in administering intrathymic injection. We thank Dr. Bing Li (Department of Pathology, Carver College of Medicine, University of Iowa, USA) for his suggestions and revising the manuscript. Without their help, the study could not have been done well. Author contributions Haojie Wu, Xiaohan Li, Chen Zhou, and Qihong Yu conducted the experiments. Shiyao Ge, Zihui Pan, and Yangjing Zhao analyzed and interpreted the data. Haojie Wu, Sheng Xia, Xiaoming Zhou, Xia Liu, and Qixiang Shao conceived and designed the experiments. Haojie Wu prepared the manuscript. As funding managers, Hui Wang and Qixiang Shao provided financial support for this study and revised the manuscript. All authors have read and approved the final manuscript. Funding The present study was supported by grants from the National Natural Science Foundation of China (grant nos. 81671541, 81273202, 81701545 and 82071738), and the Clinical Medicine Science & Technology Project of Jiangsu Province of China (grant no. BL2013024). Data availability statement All original figures and data are deposited at https://pan.baidu.com/s/1Cn11R672Fyy-jwwoXIg0Eg with the password 1r0u. Ethics approval statement All animal experimental protocols were approved by the Animal Care and Use Committee of Jiangsu University, P. R. China, and followed Chinese National Guidelines for Experimental Animal Welfare (license number: SYXK 2013-0036, UJS-IACUC-AP-2016031501). Competing Ursocholic acid Ursocholic acid interests The authors declare no competing interests. Footnotes Edited by Dr Maria Victoria Niklison Chirou Publishers note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. These authors contributed equally: Haojie Wu, Xiaohan Li, Chen Zhou. Contributor Information Hui Wang, Email: nc.ude.sju@3164000001. Qixiang Shao, Email: nc.ude.sju@xq_oahs. Supplementary information The online version contains supplementary material available at 10.1038/s41420-021-00619-5..