We quantified the amount of rDNA in the fractions by quantitative PCR (qPCR) and found very similar levels of rDNA in the high-speed supernatant fraction used for ChIP (Physique 2C), suggesting that differential fractionation of rDNA sequences cannot explain the difference in ChIP signal in mutant versus WT
We quantified the amount of rDNA in the fractions by quantitative PCR (qPCR) and found very similar levels of rDNA…