Fifty four patients with conditions other than TB were determined as the control group. test for analysis of tuberculosis were 78.1%, 72.2%, 83.3% and 65%, respectively. These guidelines for TST were 31.3%, 81.5%, 75%, and 40%, respectively. Summary Tuberculosis rapid test has better level of sensitivity than TST and may be helpful in analysis of tuberculosis like a complementary test or in epidemiological investigations. complex (7). The 6 kDa early secreted antigenic target from via chromatography and compared it with TST as the routine assay in Iran. MATERIALS AND METHODS Establishing The present study was carried out at National Study Institute of Tuberculosis and Lung Disease (NRITLD), Masih Daneshvari Hospital, located in northeastern Tehran, Iran, like a tertiary referral care center for TB and lung disease. This study was examined and authorized by the Ethics Committee of the center. Before recruiting individuals, a detailed explanation was given and an informed consent was acquired from them. NKP-1339 Study Subjects Case group included 96 consecutively admitted fresh pulmonary or extra pulmonary TB instances. Bacteriological or pathological confirmation was considered as the platinum standard for analysis of tuberculosis. Consequently, pulmonary cases were confirmed by positive sputum smear or tradition for and extra pulmonary TB individuals were confirmed by pathologic study. Fifty four individuals were selected as the control group among sequential individuals with respiratory diseases other than TB admitted to the internal NKP-1339 ward. None of them were NKP-1339 known instances of immunocompromised state, auto immune disease or under immunosuppressive therapy. Instances and settings were more than 16 years of age. Tuberculin Skin Test TST was performed for those cases and settings with 5-tuberculin unit (TU) dose of PPD (0.1 milliliter of solution) according to the protocols layed out KRAS from the World Health Corporation (WHO) (9). The reaction was go through after 48 to 72 hours. A positive test was defined from the diameter of induration more than 10 millimeters. Serological Test We used ACON Tuberculosis Quick Test manufactured by Acon? Laboratories. It is a qualitative, solid phase, two-site sandwich immunoassay for detection of anti-TB antibodies. There is a membrane pre-coated with TB recombinant antigens within the test collection region of the device. The specimen migrates upward within the membrane chromatographically by capillary action. The anti-TB antibodies, if present, react with these antigens and generate a coloured collection that means positive result. Like a technical control, colored collection will always appear in the control region indicating that appropriate volume of specimen has been added and membrane wicking offers occurred. The test can be performed with three NKP-1339 drops of any kind of these specimens including whole blood, serum or plasma. Whole blood may be supplied by venipuncture or finger stick and addition of buffer remedy is necessary. We desired venipuncture and 0.5 cc venous blood samples were collected from each patient, three drops of each sample were utilized for the test. The result should be go through at 10 minutes time. If two unique colored lines appeared, the result would be regarded as positive and if only one colored collection appeared in the control region, it was interpreted as bad. In situations where the control collection failed to display, the test would be repeated. Statistical Analysis After collecting demographic info (age, sex, and nationality), results of TST and serologic quick tests, data were analyzed using SPSS software (version 15.5, SPSS, Chicago) and level of sensitivity and specificity of the rapid test were calculated and compared to those of TST. Chi- Square test was utilized for nominal variables. RESULTS The study was performed on 96 admitted instances of tuberculosis including 88 pulmonary and 8 extra pulmonary instances and 54 admitted individuals without tuberculosis as settings. There were a total NKP-1339 of 70 males (46.7%) of both instances and settings, and 133 (88.7%) were Iranian. The mean.