Equal levels of protein per sample were packed onto 8% SDS-PAGE gels for electrophoresis and transferred onto an polyvinylidene fluoride membrane (Invitrolon; Invitrogen) using a electrophoretic transfer cell (Mini Trans-Blot; Bio-Rad, Hercules, CA)
Equal levels of protein per sample were packed onto 8% SDS-PAGE gels for electrophoresis and transferred onto an polyvinylidene fluoride…