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- Rab11-depletion results in the accumulation of immature (arrows) and abnormal (arrowheads) autophagosomes compared with the control cells
- We quantified the amount of rDNA in the fractions by quantitative PCR (qPCR) and found very similar levels of rDNA in the high-speed supernatant fraction used for ChIP (Physique 2C), suggesting that differential fractionation of rDNA sequences cannot explain the difference in ChIP signal in mutant versus WT
- For bone tissue marrow (BM) isolated MDSCs, BM haematopoietic progenitor cells extracted from tibia and femur bone fragments of C57BL/6 were treated with ACK buffer, washed and isolated using the MDSC isolation package (Miltenyi Biotec, Auburn, CA, USA) following manufacturer’s guidelines
- (a) Total cell lysates of spinal cord immune cells enriched with Percoll centrifugation were subjected to immunoblotting with the anti-KS antibody
- 7exemplifies this potential using ascites profiled and gathered from an individual patient more than a 14 wk treatment period
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